Bio-ethanol production through simultaneous saccharification and co-fermentation (SSCF) of a low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass (Pennisetum purpureum Schumach)

نویسندگان

  • Masahide Yasuda
  • Hayato Nagai
  • Keisuke Takeo
  • Yasuyuki Ishii
  • Kazuyoshi Ohta
چکیده

Efficient bio-ethanol production from napiegrass (Pennisetum purpureum Schumach) was investigated. A low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass was subjected to simultaneous saccharification and co-fermentation (SSCF), which was performed at 36°C using Escherichia coli KO11, Saccharomyces cerevisiae, cellulase, and xylanase. It was found that use of xylanase as well as the LMAA-pretreatment was effective for the SSCF. After the SSCF for 96 h, the ethanol yield reached 74% of the theoretical yield based on the glucan (397 mg g(-1)) and xylan (214 mg g(-1)) occurring in dry powdered LMAA-pretreated napiergrass.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Process design of SSCF for ethanol production from steam-pretreated, acetic-acid-impregnated wheat straw

BACKGROUND Pretreatment is an important step in the production of ethanol from lignocellulosic material. Using acetic acid together with steam pretreatment allows the positive effects of an acid catalyst to be retained, while avoiding the negative environmental effects associated with sulphuric acid. Acetic acid is also formed during the pretreatment and hydrolysis of hemicellulose, and is a kn...

متن کامل

Sustaining fermentation in high-gravity ethanol production by feeding yeast to a temperature-profiled multifeed simultaneous saccharification and co-fermentation of wheat straw

BACKGROUND Considerable progress is being made in ethanol production from lignocellulosic feedstocks by fermentation, but negative effects of inhibitors on fermenting microorganisms are still challenging. Feeding preadapted cells has shown positive effects by sustaining fermentation in high-gravity simultaneous saccharification and co-fermentation (SSCF). Loss of cell viability has been reporte...

متن کامل

Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding

BACKGROUND Simultaneous saccharification and co-fermentation (SSCF) has been recognized as a feasible option for ethanol production from xylose-rich lignocellulosic materials. To reach high ethanol concentration in the broth, a high content of water-insoluble solids (WIS) is needed, which creates mixing problems and, furthermore, may decrease xylose uptake. Feeding of substrate has already been...

متن کامل

Enhanced Ethanol Production from De-ashed Paper Sludge by Simultaneous Saccharification and Fermentation and Simultaneous Saccharification and Co-fermentation

A previous study demonstrated that paper sludges with high ash contents can be converted to ethanol by simultaneous saccharification and fermentation (SSF) or simultaneous saccharification and cofermentation (SSCF). High ash content in the sludge, however, limited solid loading in the bioreactor, causing low product concentration. To overcome this problem, sludges were de-ashed before SSF and S...

متن کامل

Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover

BACKGROUND Simultaneous saccharification and co-fermentation (SSCF) process involves enzymatic hydrolysis of pretreated lignocellulosic biomass and fermentation of glucose and xylose in one bioreactor. The optimal temperatures for enzymatic hydrolysis are higher than the standard fermentation temperature of ethanologenic Saccharomyces cerevisiae. Moreover, degradation products resulting from bi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2014